An Atomic Model of the Interferon-β Enhanceosome
نویسندگان
چکیده
منابع مشابه
An Atomic Model of the Interferon-β Enhanceosome
Protein constructs and purification For purification of NFκB:IRF-7:IRF-3, a freshly transformed colony was transferred into 10 ml LB broth containing 100 μg/ml ampicillin and grown overnight at 37°C to saturation. This overnight culture was used to inoculate 1 liter LB broth containing 100 μg/ml ampicillin and grown at 37°C to an OD600 of about 0.5. The culture was then induced with 0.4 mM isop...
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Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and NFkappaB. These factors bind cooperatively to the IFN-beta enhancer and recruit coactivators and chromatin-remodeling proteins to the IFN-beta promoter. We describe here a crystal structure of the DNA-binding domains of IRF-3, IRF-7, and NFkappaB, bound...
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Classic studies over the last two decades have made virus-induced activation of the mammalian interferon-b (ifnb) gene a prototype of eukaryotic gene regulation [1–6]. Indeed, the compact ,50 base-pair enhancer region upstream of the ifnb transcription start site is amongst the beststudied stretches of mammalian DNA, and its function in regulation of ifnb expression is considered a paradigm of ...
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ژورنال
عنوان ژورنال: Cell
سال: 2007
ISSN: 0092-8674
DOI: 10.1016/j.cell.2007.05.019